I have read that grinding plant materials causes chemical changes to occur on the broken surfaces. The seed was not ground up but used as is and seemed to work fine that way. Is DMT fat soluble? Also, could the beans be defatted chemically and then fermentation used to extract and proceed from there? Third question, the last thing worked with was a large seed, bigger than this. The last thing I extracted I did not defat I used a soxhlet, and the fat floated on the top of the solution refrigeration got the fat to coagulate into globs that could just be scooped out. I forgot.some primary literature can be found on this (extraction of DMT) at the following site:ДЈ)(optional) defat with similar volume of non-polar solvent. 'You must be,' said the Cat, 'or you wouldn't have come here.' You're mad.' 'How do you know I'm mad?' said Alice. 'Oh, you can't help that' said the Cat: 'we're all mad here.

Do this three times, combine the organic extracts, keep them.ДҐ)Remove the solvent either by stripping under vacuum (distillation setup, vacuum is not always used) or simply evaporating (don't do this where you need to breathe, and don't do this if you can do anything else.tough on the environment.Д¦) You should be left with some goo or crystals.multiple runs through the acid-base transfers may lead to a purer product if you like, but keep in mind that you are likely to lose some product in the process. Shake w/ slightly less than equal volume of non-polar/organic solvent.

naptha, DCM) in a sep funnel and shake, then discard the polar solvent.ДЈa (optional) boil up the aqueous extract with activated carbon.this is rarely done in literature because the compound of interest is often just extracted and then chromatographed.if you are having troubles getting as pure a product as you want, you might try adding a small amount of activated carbon to get rid of some of the extra heavier compounds.Д¤) Transfer DMT to organic (non-polar) solvent: bring the pH of the polar (aqueous) solution up to 10.68, by which point 99% of the DMT will be in deprotonated (neutral) form. charged) since it is 2 pH units below its pKa, which from memory is 8.68) Once the pH is at 6.68, add a similar volume of non-polar solvent (ie. Just bring the pH to 10.68 or somewhere near there (at pH 6.68 and above, 99% of the DMT is protonated (ie. Re: Mucuna pruriens (velvetbeans) and DMTДЄ typical alkaloid extraction could be used to isolate the alkaloids, although isolating just the DMT (which is what you seem to be implying) would require a further chromatography step most likely.Дў)Extract alkaloids (couple of choices here)Д¬)soak for about a week at a time with some stirringДЈ)(optional) defat with similar volume of non-polar solvent. What would be the best way to extract the DMT? And can anything be done with the 5-MeO-DMT-N-oxide? These beans are very hard even for dried beans. A friend has some Mucuna pruriens beans (velvetbeans, cowhage), which contain DMT and 5-MeO-DMT-N-oxide (also other tryptamines, L-dopa, and steroids).

Chem c3000 dmt extraction